Description
Recombinant full-length human PKAcg was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is NM_002732.
PKA C-gamma (PKAcgamma) is a third isoform of the catalytic subunit of cAMP-dependent protein kinase. It was isolated from a human testis cDNA library and was clearly derived from a gene distinct from C-alpha and C-beta and showed tissue-specific expression. Whereas at the amino acid level C-alpha and C-beta showed 93% homology, C-gamma showed only about 80% homology to both C-alpha and C-beta (1). The PRKACG gene is intronless, contains remnants of a poly(A) tail, is flanked by direct repeats, and is co-linear with the PRKACA gene(2)